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KMID : 0362719940320100733
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Journal of the Korean Dental Association
1994 Volume.32 No. 10 p.733 ~ p.740
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Effect of Serotonin of The Glutamic Acid Release From Rat Hippocampal Slice
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Abstract
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Present study was performed to clarify the effect of serotonin on the glutamic acid release employing rat hippocampal slices which appear contain most of the intrinsic and afferent circuits and to maintain the physiological characteristics seen
in
the
hippocampus italic.
Hippocampal slices were prepared and pre-equilibrated in Krebs0bicarbonate medium(KBM, pH7.4) for 1hr at 37℃. Pre-equilibrated slices were incubated in fresh KBM and then potassium-containing KBM for 5min period. Basal and potassium induced
glutamic
acid release were determined from recovered medium by HPLC. After 30min resting period, slices were reincubated in serotonin-containing KBM and serotonin plus potassium-containing medium consecutively for 5min period each to investigate the
effect
of
serotonin on basal or potassium-induced glutamic acid release from hippocampal slices.
@ES The observed results were as follows:
@EN 1. The release of glutamic acid induced by the first and second 5-min exposure of 50mM potassium was 145.9±13.2 nmol and 125.6±12.8 nmol, respectively.
When compared with released amounts of glutamic acid during the corresponding spontaneous periods, these were 5.2 and 5.4-fold increase respectively.
2. Serotonin(1-100μM) had no significant effect on the spontaneous release of glutamic acid.
3. Serotonin)1-100μM) inhibited potassium-induced glutamic acid release in dose-dependent manner.
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KEYWORD
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